Abstract
An HPLC method was developed for the monitoring of pitavastatin in pharmaceuticals using C18 column with detection wavelength at 278 nm. Phosphate buffer, acetonitrile and methanol with the ratio of 30:40:60 was selected as best mobile phase with pH 7.4 and pitavastatin was detected at 278 nm with flow rate of 1 mL/min. The retention time was 3.728 minute while the linearity was determined in the range of 300 to 800 ng/mL with 0.9997 coefficient of correlation. Percentage recoveries in terms of accuracy were in the range of 91.83 to 97.66%. Assay result of pitavastatin in self nanoemulsifying drug delivery system (SNEDDS) was 97.35 %. Method was successfully applied for determination of pitavastatin in developed SNEDDS due to its highest validity.