Abstract
This study was conducted to obtain a high frequency regeneration from Basmati 385 and Super Basmati, which is a pre-requisite for transformation protocol. Seed was the explant source used in this study. Callus induction was obtained from N-6 media with 2 mg l(-1) 2,4-D. Super Basmati exhibited high callus induction efficiency (93.3%) followed by Basmati 385 (90.2%). Twenty one days old maintained calli when transferred on MS medium with different combinations of auxin-cytokinin for regeneration showed 90% frequency of plant regeneration for Super Basmati with NAA 1 mg l(-1) and BAP 2.5 mg l(-1) and 83% for Basmati 385 on MS medium supplemented with NAA I mg l(-1) and BAP 5 mg l(-1).