Abstract
Genetic typing of the intronic (CA)n loci within the
dystrophin gene was exploited for linkage analysis and diagnosis of carrier status.
IP-RP-HPLC was developed for genotyping of 98 chromosomes.
The typing of 2-bp STRs with mean SD of ±
0.43 bp ensured an allele's identity with 99.7% confidence.
The automation enabled the accurate sizing of unlabeled alleles with an excellent reproducibility of 100% with significant labor saving and reduced reagent costs.