Abstract
10.1601/nm.1335 Mlalz-1 (INSDC =
ATZD00000000
) is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen-fixing nodule of
Medicago laciniata
(L.) Miller from a soil sample collected near the town of Guatiza on the island of Lanzarote, the Canary Islands, Spain. This strain nodulates and forms an effective symbiosis with the highly specific host
M. laciniata
. This rhizobial genome was sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) sequencing project. Here the features of 10.1601/nm.1335 Mlalz-1 are described, together with high-quality permanent draft genome sequence information and annotation. The 6,664,116 bp high-quality draft genome is arranged in 99 scaffolds of 100 contigs, containing 6314 protein-coding genes and 74 RNA-only encoding genes. Strain Mlalz-1 is closely related to 10.1601/nm.1335
10.1601/strainfinder?urlappend=%3Fid%3DIAM+12611
T
, 10.1601/nm.1334 A 321
T
and 10.1601/nm.17831
10.1601/strainfinder?urlappend=%3Fid%3DORS+1407
T
, based on 16S rRNA gene sequences. gANI values of ≥98.1% support the classification of strain Mlalz-1 as 10.1601/nm.1335. Nodulation of
M. laciniata
requires a specific
nodC
allele, and the
nodC
gene of strain Mlalz-1 shares ≥98% sequence identity with
nodC
of
M. laciniata
-nodulating 10.1601/nm.1328 strains, but ≤93% with
nodC
of 10.1601/nm.1328 strains that nodulate other
Medicago
species. Strain Mlalz-1 is unique among sequenced 10.1601/nm.1335 strains in possessing genes encoding components of a T2SS and in having two versions of the adaptive acid tolerance response
lpiA-acvB
operon. In 10.1601/nm.1334 strain 10.1601/strainfinder?urlappend=%3Fid%3DWSM+419,
lpiA
is essential for enhancing survival in lethal acid conditions. The second copy of the
lpiA-acvB
operon of strain Mlalz-1 has highest sequence identity (> 96%) with that of 10.1601/nm.1334 strains, which suggests genetic recombination between strain Mlalz-1 and 10.1601/nm.1334 and the horizontal gene transfer of
lpiA-acvB
.