Abstract
Ultra-thin two-dimensional (2D) materials have attained huge interest for biosensing applications because of their strong electrostatic coupling with target molecules such as spike proteins and DNA. One such 2D material is graphene, which is extremely thin and flexible and has a strong non-covalent interaction with the supporting constructs needed to detect biomolecules. This work aimed to develop a way to efficiently functionalize the surface of 2D material using a pyrene-based supporter construct to detect the target protein. For this purpose, high-quality, pristine graphene was grown via the chemical vapor deposition (CVD) method and transferred over the Si/SiO2 substrate for its functionalization using our engineered pyrene-lysine-based supporter construct (PLB). The construct was synthesized using the solid-phase peptide synthesis (SPPS) method and utilized to functionalize the graphene-channel-based field-effect transistor (FET) device via non-covalent pi-pi stacking interaction. The optimum concentration of the functionalized PLB was evaluated via atomic force microscopy (AFM), Raman spectroscopy, and real-time electrical measurements. The characterization techniques successfully provide an overview of the effect of the concentration of PLB used for functionalization. Moreover, the performance was tested and compared in terms of the percentage response of the device generated after the detection of various concentrations of the streptavidin protein. This research could be useful in determining how to functionalize any 2D material by designing a supporter construct without material degradation and owing to over-stacking or bypassing surface screening effects.