Abstract
Horse radish peroxidase (HRP) has been electrochemically entrapped into perchlorate (ClO4-) doped polyaniline (PANI) film deposited onto indium-tin-oxide (ITO) coated glass plate. This HRP-PANI-ClO4-/ITO bioelectrode characterized using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), UV-Visible spectroscopy, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) techniques has been utilized for estimation of hydrogen peroxide (H2O2). This H2O2 sensor exhibits response time of 5 s, linearity from 3 to 136 mM, sensitivity as 0.5638 mu AmM(-1)cm(-2) with linear regression of 0.985. The value of the Michaelis-Menten constant (K-m) has been obtained as 1.984 mM. Copyright (C) 2009 John Wiley & Sons, Ltd.