Abstract
Background: Molecular marker based cancer diagnosis gaining more attention in the current gcnomics era, So, Uspb1 and Tp53 gene characterization and their mRNA expression might be helpful in diagnosis and prognosis of cat mammary adenocarcinoma. It will also add informationin comparative cancer genetics and genomics.
Objectives: Eight tumors of Siamese cats were analyzed to ascertain germ-line and tissue-specific somatic DNA variations of Hspb1 and Tp53 genes along with the ectopic differential expressionin tumorous and normal tissues were also analyzed.
Materials and Methods: Tumorous tissues and peripheral blood from mammary adenocarcinoma affected Siamese cats were collected from the Pet center-UVAS. DNA and RNA were extracted from these tissues to analyze the Hspb1 and Tp53 DNA variants and ectopic expression of their mRNA within cancerous and normal tissues.
Results: Exon 1 and 3 revealed as hotspots in Hspb1 gene. The 5'UTR region of the exon1 bearsix mutation including 3 transitions. 2 transversion and one heterozygous synonymous transversion in two samples at locus c.34C>C/A. Exon 3 has 1 transversion at c.773A>A1T. 3'UTR of this exon harbor two point mutationsat 1868A>T and 2193C>T loci. lntron 2 has two alterations at 1490C>C/T and GTCT4del at 1514. Overall up-regulationof Hspb1 gene was observed. While exons 3, 4 and 7 of Tp53 harbor asingle variationat c.105A>AiG, c.465T>T/C and c.859G>T respectively. The locus c.1050G>G/A in exon 9 is a heterozygous (G/A) in 3 samples and homozygous (Cr) in 2 other tumours. Introns 3, 5, 7 and 9 harbor 3, 4, 2 and 7 altered loci respectively. Sixty percent of cancers showed up-regulated trend of Tp53 gene.
Conclusions: Tumor specific mutations and ectopic expression of Hspbl and Tp53 genes might be helpful in the diagnosis of the mammary lesions and endorse their involvement in cat mammary neoplasm.