Abstract
Aims: This Study was performed to evaluate the quantitative distribution of mannitol (model hydrophilic molecule) and corticosterone (model lipophilic molecule) in human skin following the application of different vesicular systems (liposome, ethosome and invasome).
Methodology: Vesicular systems were prepared by thin film hydration method and particle size, particles distribution, zeta potential and in vitro human skin penetration and skin deposition were evaluated.
Results and Discussion: Results showed that all of the investigated vesicular systems had almost nanometric size ranging from 67.46 +/- 0.25nm and 108.3 +/- 0.95nm with low polydispersity (PDI < 0.2). All vesicular systems exhibited negative zeta potential. In vitro skin deposition of corticosterone showed that invasome had significant lower accumulation than ethosome and liposome in SC (by similar to 5 and 4 folds respectively) while showed only lower accumulation than liposome in epidermis. Ethosome showed insignificant higher accumulation than CL-CS in both SC and epidermis. Mannitol data revealed that ethosome and liposome showed significant higher accumulation than invasome (by similar to 15 and 4.5 folds respectively) while showed liposome slight insignificant higher accumulation than liposome in SC. Going deeper into epidermis and dermis, vesicular systems and ethanolic solution improved accumulation when compared with buffered solution.
Conclusion: In sum, drug deposition in and penetration through skin is not only influenced by formula in which the drug is incorporated (though it is important factor) but also affected by another factors such as partition coefficient of the drug.