Abstract
Shilajit is a blackish-brown exudation, consisting of organic substances, metal ions and minerals, from different formations, commonly found in the Himalayan region (1000-3000 m) from Nepal to Kashmir. Shilajit can also be collected throughout the mountain regions in Afghanistan, Bhutan, China, Ural, Baikal, Sayalcacasus and Atlai at altitudes between 1000 to 5000 m. The major physiological action of shilajit has been attributed to the presence of bioactive dibenzo-?-pyrones together with humic and fulvic acids, which act as carrier molecules for the active ingredients. In this work, the aim was to extract humic acid from Shilajit from various sources and characterised these humic acids based on their physico-chemical properties, elemental analysis, UV/Vis and FTIR spectra, X-ray diffraction pattern and DSC thermograms. The spectral features obtained from UV/Vis, FTIR, XRD and DSC studies for samples of different origins showed a distinct similarity amongst themselves and in comparison to soil humic acids. The surfactant properties of the extracted fulvic acids were investigated by determining the effect of increasing concentration on the surface tension of water. The study demonstrated that humic acids extracted from shilajit indeed possessed surfactant properties.