Abstract
Fermentative hydrogen production was carried out using
Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). This work investigates the effects of initial substrate concentration, initial medium pH, and temperature. The hydrogen yield was about 3.1
mol (mol glucose)
−1 when starting with an initial glucose concentration of 10
gl
−1 and initial a pH of 6.0
±
0.2 at a temperature of 37
°C. The volume of hydrogen produced decreased when higher initial glucose concentrations were applied. The most suitable conditions for hydrogen production in a batch reactor were observed at initial pH 6.0
±
0.2 and 37
°C.