Abstract
The potential benefit of selenium nanoparticles (SeNPs) and vitamin. E (vit. E) that they are potent antioxidants. Acrylamide (ACR) is found in the starchy foods that had been heated for long periods in high degrees above 120 degrees C. The current aim of the study was to elucidate the anti-hyperlipidemic and hepatoprotective effects of either SeNPs and/or vit. E against ACR. SeNPs were characterized by SEM, EDX,TEM and they proved their spherical nanostructure, Also zeta potential value was -22.9 mv with average particle size was 132 nm and all these characterizations proved the high stability of the used SeNPs. Seven groups of male mice were used: group I was control, group II ACR (500 mg/Kg), group III was treated with SeNPs (1.7 ppm), IV group was received vit. E (100 mg/Kg) and groups V,VI received ACR plus SeNPs and ACR plus vit. E, group VII was given combination of SeNPs and vit. E plus ACR. We monitored the effect of either SeNPs or vit. E against ACR hepatic toxicity by studying the alteration in the hepatic enzyme activities [alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH)], lipid profile alteration as well as evaluation of the antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT), Myloperoxidase (MPO), Xanthine oxidase (XO) and glutathione reductase (GRx) and evaluation of the marker of lipid peroxidation (MDA). Histological and transmission electron microscope of the liver tissues were examined. The mice that were administrated ACR only had a high significant elevation in the hepatic enzymes with severe alterations in the lipid metabolism. The findings clarified the efficacy of SeNPs and vit. E as a hepatoprotectants and potent antioxidant on ACR by improving the liver enzyme activities along with the lipid profile, as the activities of ALT, AST, and LDH were significantly reduced with elevation of antioxidant enzymes (SOD,CAT and GRx) with reduction in level of MDA. Histological sections and ultrastructural examination revealed a highly congestion and fibrosis of hepatic tissues in ACR-group while ACR combined with SeNPs and/or vit. E showed restoration of almost normal hepatic structures as the same TEM sections. In conclusion, SeNPs and/or vit. E were proved to be a potential hepatoprotective, hypolipidemic agents and enhance antioxidant capacities as they significantly ameliorate the hepatotoxicity induced by ACR.