Abstract
Carbohydrates are one of the largest and most important food complexes added to the nutrient medium for the growth and development of the tissues of jojoba plant and its propagation in vitro. Five types of artificial sugars were studied with three gradient concentrations to determine the best. In addition, the study also investigated the possibility and efficacy of replacing sugars as an artificial source of carbon and industrial energy with natural ones such as natural extract (date syrup) as a good, suitable, cheaper, available and known in the Saudi Kingdom, so the study also investigated date syrup (dibs) as a natural source of carbohydrates. In general, the data showed that the best rate of growth and development of plantlets was with sucrose 30, then 45 g/l nutrient medium, followed by glucose then fructose under the same concentration. Whereas the results found that, the lowest growth was with the explants grown in media containing any concentration of mannitol or sorbitol. On the other hand, the results clarified that the best for the growth and development of the cultivated jojoba tissues was with the culture medium that contains the natural extract (Dibs) at a concentration of 30 g/l. This indicates that date syrup can replace sucrose, thereby reducing the total costs of the micropropagation of jojoba in vitro.