Abstract
As an anticancer medication, L-asparaginase enzyme from bacteria has been applied as an efficient therapeutic agent for acute lymphoblastic leukemia (ALL) treatment. Current investigation was designed to optimize and partially purify the L-asparaginase from Bacillus safensis. Bacterial growth conditions such as nutrition, pH. temperature, inoculum size and incubation periods have been examined for optimal L-asparaginase production. Findings revealed that, maximum L-asparaginase activity from Bacillus safensis was 123.53 LT/minlml that obtained after 48h at 35 2 C. of incubation in shaking incubator (150 rpm). The best tested culture (inoculum size, 30%) was supplemented with L-asparagine (0.75%) for B. safensis in the presence of yeast extract (0.25 0) without carbon source at pH 8. Therefore, he most positive significant independent factors influencing enzyme production are time, temperature, pH, inoculum size, and agitation speed. The crude enzyme was extracted from B. safen.cis free cell that filtered and partially purified by ammonium sulphate (80%). In conclusion: Bacillus safensis showed its ability as promising candidate to produce L-asparaginase as well as enzyme activity was maximized at nutrition medium without carbon sources.