Abstract
Cancer is a mortal disease needs a potent therapy with low side effects. So, the aim of this study is to evaluate the antitumor efficiency of different concentrations of silver nanoparticles (AgNPs) biosynthesized by seaweeds (Ulva fasciata, Corallina elongate, Gelidium crinale, Laurencia obtusa, Cystoseira myrica and Turbinaria turbinata) on Ehrlich Ascites Carcinoma (EAC) in vitro. The most cytotoxic silver nanoparticles synthesized by one of our studied algae would be examined by TEM, SEM, EDAX, X-ray and FT-IR to determine their characterizations. Silver nanoparticles were synthesized by the reduction of silver nitrate in the algal aqueous extracts. The green synthesis of AgNPs through algal extracts was indicated by a colour change and UV spectroscopy. Different concentrations of AgNPs were used to study their cytotoxic activity against EAC in vitro. Trypan blue exclusion test showed a decrease in EAC cells viability by increasing AgNPs concentration in all tested algae. The maximum inhibition percentage of EAC cells were (94 and 99%) with 98 mu g/ml AgNPs biosynthesized by Ulva fasciata and Turbinaria turbinata respectively. Silver nanoparticles biosynthesized by Turbinaria turbinata were the most cytotoxic against EAC in vitro. Characterization of the biosynthesized AgNPs by Turbinaria turbinata confirmed biosynthesis of AgNPs with spherical shape and size varying from 8-16 nm.