Abstract
Introduction: Reactive oxygen species and nitric oxide play a major role in the physiopathology of inflammatory diseases, neurodegenerative disorders, autoimmune pathologies and immunodeficiency. With this understanding targeting oxidative stress could be a promising approach in new drug discovery. Methodology: Therefore, new compounds that were isolated from methanolic extract of leaves of wild strawberry, Fragaria nubicola were tested for its in-vitro anti-inflammatory activity against the free radical's generation during the process of respiratory burst by luminol enhanced chemiluminescence technique. The effect on superoxide and nitric oxide production was measured in cell culture supernatant by cell based spectrophotometric assay. The cytotoxicity analysis was done using a fibroblast cell line by MTT assay. Results: a current study has demonstrated the potential of selected compounds being an effective immunomodulating agent. These compounds (1-5) were found to be significantly inhibiting the myeloperoxidase dependent free radical generation with an IC50 value of 4.6 +/- 1.3, 5.7 +/- 1.6,<1, 2.3 +/- 0.01 and <1 mu g/mL respectively. As compare to ibuprofen, which showed IC50 of 43.4 +/- 0.9 mu g/mL. However, the compounds did not exert any effect on suppressing the myeloperoxidase independent pathway and not able to suppress superoxide anion. For nitric oxide production, the compounds 1 and 5 showed moderate activity with % inhibition of 35.1 and 38.3%, respectively. None of the compound showed cytotoxicity up to 25 mu g/mL concentration. Conclusion: Results suggested that the new compounds were found to be selective inhibitors of myeloperoxidase dependent pathway of a respiratory burst with no cytotoxic effect. Revealing them as promising drugs, specifically targeting oxidative stress associated pathologies.