Abstract
Present investigation was carried to find out efficacy of crude Aloe vera leaf gel in protecting hepatic cells against the oxidative stress mediated by varying concentrations of APAP (AAP) during different periods of time. APAP is non toxic in therapeutic doses but imparts toxicity when given singly in high doses. Aloe vera is a medicinal herb and the leaf gel is widely utilized for treating diseases due to medicinal properties associated with it. Hence in the present investigation, the leaf gel was studied. In vitro rat liver slice model was used to study and evaluate the hepatocyte protection offered by the crude gel of Aloe vera leaf. AST, ALT, ALP levels in the medium were studied as the markers of hepatocyte damage along with Glutathione and TBARS in the liver tissue slices. No significant toxicity was observed at the end of one hour of incubation, while there was dose dependent toxicity induction and leakage of enzymes in the media in presence of increasing doses of APAP and increasing time of incubation. The higher concentration of Aloe vera was more effective to protect the cells against APAP induced toxicity amongst the two different concentrations of Aloe vera tested for their protective efficacy. The toxicity studies of Aloe vera leaf gel showed that Aloe vera leaf gel is safe for hepatocytes and no toxicity outset was observed in terms of enzyme leakage and TBARS. Aloe vera leaf gel is effective in protecting the liver cells against APAP mediated oxidative stress and related damage in terms of the parameters studied in the present project.