Abstract
Monocrotophos (MCP), generally famous as azodrin, is widely applied in agricultural work all over the world. However, few data is available on its short-term cytotoxic effects on human keratinocyte (HaCaT) cells. The aim of this study was to estimate toxicity and its mechanism in HaCaT cells. In this study, we found cytotoxic activity and oxidative stress produced in HaCaT cells due to exposure of MCP for 24 and 48 hours. MCP (0, 100, 300, and 600 ng/ml) produced dose and time dependent cytotoxicity in HaCaT cells, which was determined by MTT and NRU assays. MCP induced oxidative stress, demonstrated by elicits of reactive oxygen species (ROS) generations, lipid peroxides (LPO) and declination of glutathione (GSH) in HaCaT cells. Due to exposure of MCP, chromosome condensation was found in HaCaT cells, which is hallmark of apoptosis was indicated by DAPI (4',6-diamidino-2-phenylindole). Maximum, DNA single strand breakage was found at maximum dose of MCP for 48 hour. This study suggested that MCP induce cytotoxicity and apoptosis in HaCaT cells, which is likely to be facilitated via ROS production and oxidative stress..