Abstract
36 Bacillus native strains were isolated and identified using 16s gene sequencing analysis. Universal primer of 16s rRNA gene was used and PCR amplifications were done with all DNA isolation from all isolated strains. Mixture of non-identical 16S rRNA amplicons products produced and bioinformatics data analysis of all yielded variable consensus sequence were done using different software programs. The data analysis of sequences displayed some sequence variability occurs among the multiple 16S rRNA genes. Bioinformatical analysis revealed that the nucleotide sequences obtained from the isolates were different homologous to the known DNA sequence using NCBI Blast software. Results revealed that the obtained Bacillus isolated strains were belonging to17 different species. No noticeable base frequencies variation were observed in base frequencies for all the 39 isolates except B.simplex strains in in cytosine base. Results of phylogenic analysis revealed that all isolated strains are clustered analysis in 2 large clusters. The Polymorphism and Genetic Diversity among the isolates were also done using Clustalx and Maft build analysis, the monomorphic and polymorphic sits of all strains also analyzed.Further study for complete genome sequence analysis will be needed. The bioinformatics tools were succeeded in analyze all our native bacillus strains.