Abstract
Our study is considered to attempt reducing the immune-toxic and antioxidant impacts of exposure to fipronil (FP) on Nile tilapia, Oreochromis niloticus using the beta-glucan (beta G). Two hundred and seventy fingerlings of Nile tilapia were divided randomly into six groups (45 tilapias of each, in 3 replicates): group I control (CT) group nourished on a basal diet. Group II (beta G) nourished a basal diet supplemented with 0.4%beta G. Groups III (1/20 FP) and V (1/10 FP) was exposed to 1/20 and 1/10 of the 96 h LC50 of FP in water and nourished the basal diet respectively. Groups N (1/20 FP + beta G) and VI (1/10 FP + beta G) were exposed to 1/20 and 1/10 FP concomitantly with 0.4% beta G supplementation for 90 successive days. Growth performance metrics were higher in beta G group than CT. While those metrics were fallen at exposure to 1/20 or 1/10 FP. Supplementation with beta G elevated the IgM and lysozyme levels.Whereas, tilapias exposed to FP only at different concentration showed lowering of those compared to CT. Supplementation with beta G was effectively augmented IgM and lysozyme in 1/20 FP exposed tilapias. Furthermore, in a minor grade at 1/10 FP exposed tilapias. Exposure to FP increased the activities of hepatic markers chiefly at 1/10, however the supplementation was successfully improved these markers. There was imbalance of cortisol level at FP exposure where, beta G combining to FP alleviate this disparity. There was fallen in LDH, MDH and FDPase in beta G tilapias where continuing raise in 1/10 FP followed by 1/20 FP. beta G supplementation raise the level of GSH, without significant variations in MDA conversely occurs in FP alone. Genes expression of beta G caused raise of both GPx and GR, without fluctuations in CAT and SOD. Exposure to FP diminishes all evaluated antioxidant genes. It could fulfilled that supplementation with beta G successfully alleviated the immune-toxic and antioxidant impact of FP in tilapias.