Abstract
The JB-4 embedding system has become an important allernative to paraffin and frozen tissue sectioning when increased resolution is required or when there is a need to preserve sensitive antigens. Although methacrylate embedding has many advantages, impracticalities in routine sectioning have kept it from achieving wide application. For instance, the sectioning of methacrylate-embedded tissues requires a special microtome and the use of glass knives. In Present work, an adapted JB-4 approach is used for the analysis of lymphocyte nmkers. Tissue sections are cut on a standard rotary microtome with a carbide blade and a modified chuck. Tissues are both fixed and dehydrated in acetone at −20°C and then infiltrated with JB-4 resin. Thin sections, 2 to 4 microns thick, were immunohistochemically stained for murine class II MHC and B7-2 using the ABC method Sections prepared by this method showed antigen specific staining with improved histologic and cytologic detail when compared with frozen sections (The J Histotechnol 26:53, 2003)
Submitted July 19,2002; Accepted with revisions November 29, 2002