Abstract
Laser ablation was used to create novel nanocomposites of Chitosan-Poly (Vinyl Pyrrolidone) (PVP) blend incorporating graphene oxide nanoparticles (GONPs) to be utilized in microbial applications. The change in the structural, and optical properties of pure Chitosan-PVP after incorporated by graphene oxide have been investigated. XRD results confirmed the incorporation of GO into Chitosan/PVP blend and FT-IR results confirmed the interaction between Chitosan-PVP and GO NPs. UV data was confirmed that Chitosan/PVP/GO NPs after laser ablation time 10 min has lower band gap energy compared to other samples. The antimicrobial behavior of Chitosan/PVP/GO NPs at two times of laser ablation (5 min and 10 min) were performed against E
. coli
,
P. aeruginosa
,
S. aureus
,
B. subtilis
, and
C. Albicans
. The antimicrobial activity shows enhancement in the inhibition zones, it was enhanced from 5 ± 0.98 to 13 ± 0.72 for
E. coli
, 6 ± 0.56to 15 ± 0.84 for
P. aeruginosa
, 7 ± 0.73 to 17 ± 0.26 for
S. aureus
, 4 ± 0.89 to 15 ± .07 for
B. subtilis
, and 3 ± 0.54 to 8 ± 0.76 for
C. Albicans
. Chitosan/PVP/GO NPs significantly improve antimicrobial effects. The prepared compositions could be suggested after further study to be applied in microbial applications.