Abstract
The main goal of this experiment has been aimed to achieve a method for improving secondary metabolites such as phenolics beside photosynthetic pigments content of Origanum vulgare (family: Lamiaceae) callus plants. This investigation has been studied the usage of two bioelicitors as thiamine and coconut water. Our results revealed that the different concentrations of thiamine and coconut milk affected callus fresh weight (g) of O. vulgare. Both treatments increased callus fresh weight than the control. They are also affecting photosynthesis pigments but, the increment in case of (coconut milk) almost was not significant compared with thiamine which absolutely increased fresh and dry weight, total phenolics and photosynthetic pigments content by the level of 0.5 g/L.