Abstract
Tabernaemontana ventricosa (Apocynaceae) a latex-bearing plant is used in traditional medicine for its therapeutic benefits in reducing fever and hypertension and wound healing. Due to limited information on the plant's pharmacological activities, this study aimed to investigate the antioxidant potential of the leaf, stem, and latex extracts of T. ventricosa, using the Folin-Ciocalteu (total phenolics), aluminum chloride colorimetric (total flavonoids), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) assays. The cytotoxic activity was evaluated in the human HEK293 (embryonic kidney), HeLa (cervical carcinoma), and MCF-7 (breast adenocarcinoma) cell lines using the MTT assay. The latex extracts possessed the highest total phenolic content (115.36 +/- 2.89 mg GAE/g), followed by the stem hexane extracts (21.33 +/- 0.42 mg GAE/g), the chloroform leaf (7.89 +/- 0.87 mg GAE/g), and the chloroform stem (4.69 +/- 0.21 mg GAE/g) extracts. The flavonoid content was substantially high ranging from 946.92 +/- 6.29 mg QE/g in the stem hexane, 768.96 +/- 5.43 mg QE/g in the latex, 693.24 +/- 4.12 mg QE/g in the stem chloroform, and 662.20 +/- 1.00 mg QE/g in the leaf hexane extracts. The DPPH assays showed the highest percentage of inhibition at 240 mu g/mL, for the stem hexane (70.10%), stem methanol (65.24%), and stem chloroform (60.26%) extracts, with their respective IC50 values of 19.26 mu g/mL (stem hexane), 6.19 mu g/mL (stem methanol), and 22.56 mu g/mL (stem chloroform). The FRAP assays displayed minimal inhibition ranging from 4.73% to 14.40%, except for the latex extracts which displayed moderate inhibition at 15 mu g/mL (21.82%) and substantial inhibition at 240 mu g/mL (98.48%). The HeLa and MCF-7 cell lines were the most sensitive to the extracts, with the hexane, chloroform, and methanol leaf and stem, and latex extracts significantly affecting the percentage cell survival. Overall, the various parts of T. ventricosa exhibited strong antioxidant activity correlating to its cytotoxicity. Further studies should focus on the isolation of specific antioxidant compounds that could be investigated for their anticancer potential.