Abstract
Thirty White Leghorn hens were divided into 3 groups. Inactivated S. aureus was administered to groups H1, H2 and H3 at the dose rate of 10(6) CFU/ml, 10(9) CFU/ml and 10(12) CFU/ml on days 0, 7 and 21, respectively. Antibody titers were determined by IHA. On day 7 to 21 post inoculation of hens with bacterin-toxoid, the antibody titers of groups H1, H2 and H3 were minimum 64, 78.8 and 97 and maximum GMT of 128, 157.7 and 194, respectively. The protein content of egg yolk immunoglobulins was 7.05mg/ml estimated from bovine serum albumin standard curve. For in vivo evaluation, 40 S. aureus mastitic buffaloes were selected on basis of SFMT and divided into 4 groups (Group A and B = clinicaly S. aureus mastitis C and D = subclinicaly S. aureus mastitis). Groups A and B received intramammary infusions of egg yolk antibodies and antibiotic at the dose rate of 10 ml/affected quarter, respectively. Similarly, groups C and D received the same treatment. Evaluation criteria include microbiological cure rate, clinical cure rate, SFMT and milk yield. The milk yield of 90% and 40% buffaloes was found increased in the groups that received egg yolk antibodies and antibiotic, respectively. Similarly, clinical and microbiological cures rates were 50% better in the egg yolk treated buffaloes than antibiotic treated buffalo groups. On basis of SFMT, 80% and 40% buffaloes recovered from mastitis in the egg yolk treated and antibiotic treated buffalo groups, respectively.