Abstract
The coulometric efficiency (
C
eff) of an amperometric detector integrated on PDMS/glass capillary electrophoresis microfluidic device (microchip) has been enhanced by in-channel electrochemical modification. In-channel electrochemical deposition of gold particles was performed in order to vertically increase the surface area of the Au sensing microelectrode. The roughness of the electrodes was characterized using scanning electron microscopy and profilometric analysis. The degree of electrode modification was also characterized by roughness factor determination. Separation processes including detection potential was optimized and the analytical performance of the microchip was tested using a mixture of dopamine (DA) and catechol (CA). The modified electrochemical detector provided well-resolved separation of DA and CA in less than 60
s with enhanced sensitivity; no peak broadening was observed. The limit of detection using in-channel modification of working electrode for DA and CA are 60 and 110
nM, respectively. Thus, in-channel electrochemical deposition of metallic particles should be used to enhance the
C
eff of integrated amperometric detection of analytes with good redox properties in order to obtain lower LODs.