Abstract
•Green Synthesized Nickel Oxide Nanoparticles using Extract of Terminalia chebula.•In vitro Cytotoxicity, MMP and ROS Activity of NiO NPs against MCF-7 Cells.•NiO NPs decreased the mitochondrial membrane potential (MMP) towards MCF-7 cells.•NiO shows minimal toxicity at 20–80 μg/mL towards non-tumourigenic MCF-10A cells.
Nanotechnology has been offered the prospect for the development of novel nanomaterials with countless potential applications in natural sciences and clinical pharmaceutical. This study described the analysis of green synthesized nickel oxide nanoparticles (NPs) using the extract of Terminalia chebula. The Green synthesized NiO NPs were characterized by Transmission Electron Microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-rays Analysis (EDAX) and ultraviolet-visible spectroscopy (UV–Vis). Green synthesized NiO NPs displayed the toxicity to breast cancerous cells in a dose-dependent way from 0 to 100 µg/mL showing noticeable cell viability, Reactive oxygen species (ROS) activity and liberating of mitochondrial membrane potential (MMP). The statistical scrutiny was also done on the experimental outcomes to check the value and precision of the effects, with p-values <0.05 selected as significant. The planned approach deliberates the NiO NPs as a function of phenolic extracts of T. chebula with vast potential for several biological and biomedical applications.