Abstract
The present study reports the optimized silver nanoparticles (AgNPs). They were tested against, three wound infecting gram-positive and gram-negative pathogenic bacteria viz.,
B. subtilis, S. aureus, M. luteus, E. coli, K. pneumoniae
and
P. aeruginosa
at 10 µg concentration. In the antibacterial activity of optimized AgNPs,
P. aeruginosa
was found remarkably sensitive to the AgNPs with 20.3 ± 1.86 mm of inhibition zone. The standard antibiotics of streptomycin, gentamycin, ampicillin and erythromycin at 10 µg, when tested against the bacteria, which revealed that gentamycin showed high antibacterial activity against all the six wound infecting pathogenic bacteria. The MIC and MBC concentrations evaluated against the bacteria showed were 4.0 ± 1.00 µg/mL and 6.3 ± 0.47 µg/mL for
S. aureus
, respectively. The normal morphology of tested bacteria was changed when treated with optimized AgNPs as evidenced under TEM analysis. The optimized AgNPs impregnated on cotton fabrics showed high antibacterial activity against tested bacteria. Thereafter there was a gradual decrement in antibacterial activity against the bacteria at 2nd, 3rd and 4th wash. The cytotoxicity effect of optimized AgNPs treated normal cell (Vero) recorded a maximum IC
50
value 20 µg/mL. Our research outcome opens up new improved antimicrobial composition in pharmaceutical and medical sectors.