Abstract
Three types of common and one commercial antifungal drugs were tested in vitro for assessing their effectiveness against dermatophytic fungi Trichophyton verrucosum using a microdilution assay of the NCCLS (M38-P) standard for filamentous fungi with slight modifications. Four agents of antifungal drugs in different concentrations defined as local mixture [consisting of cupric sulfate and calcium oxide (quicklime) by a mixture proportion of 1:1 in concentration of 80.0 mg mL(-1)], Hydrogen Peroxide mixture in concentration of 180.0 mg mL(-1), 10% Formaldehyde mixture in concentration of 39.0 mg mL(-1) and Amphotericin B (AMB) in concentration of 2.0 mg mL(-1). The results showed that there is a discrepancy among the antifungal drugs on the impact of dermatophytic fungi Trichophyton verrucosum. The order of effectiveness of four antifungal drugs against dermatophytic fungi was AMB with a Minimum Fungicidal Concentration (MFC) (MFC90% = 2.0 mu g mL(-1))> 10% Formaldehyde mixture with a concentration of (MFC90% = 3.9 mu g mL(-1))> Hydrogen Peroxide mixture with a concentration of (MFC90% = 18.0 mu g mL(-1))> the local mixture with a concentration of (MFC90% = 80.0 mu g mL(-1)). In conclusion, AMB with a Minimum Fungicidal Concentration (MFC) (MFC90% = 2.0 mu g mL(-1)) is the most effective drug against dermatophytic fungi under the climatic condition of AL-Ahsa, Saudi Arabia.