Abstract
Streptomyces spp. ERI-3 was isolated from forest rock soil sample of the Western Ghats, Tamil Nadu, India. Physiological, biochemical and 16S rRNA studies strongly suggested that this isolate belonged to Streptomyces. Preliminary screening revealed that the isolate was found to be active against bacteria and fungi. The growth parameters such as carbon source, pH and temperature were optimized. Nutritional and cultural conditions for the production of antimicrobial metabolite by this organism under shake-flask conditions have been optimized. The extracts were obtained by partition chromatography using hexane, chloroform, ethyl acetate and ether. The antimicrobial. activity was assessed using broth microdilution technique. Ethyl acetate extract showed good antibacterial activity. Minimum Inhibitory Concentration (MIC) was determined as 0.25 mg/ml. for Staphylococcus aureus, Staphylococcus epidermidis, Xanthomonas spp. and Candida albicans. (C) 2008 Elsevier Masson SAS. All rights reserved.