Abstract
The aim of the present study was to determine the capability of Gypsophila paniculata for plant regeneration using two explants (shoot tips and lateral buds) on four medium protocols and subsequently, to find the best explant source linked with the optimum medium conditions for the high potentiality of shoot formation. The results indicated that medium protocols and medium protocols X explants interactions had insignificant effects on callus induction. On the other hand, explants gave high significant differences in callus induction. The bud explants gave higher values (16.66%) in callus induction compared to shoot tip explants (5.82%). The results also revealed that medium protocols caused highly significant variations in shoot formation, while, medium protocols X explants interaction gave high significant variance. On the other hand, explant did not cause any significant variance. The protocol A, which was MS supplemented with 0.5 mg/L BA and 0.5 mg/L NAA, was highly significantly superior to the other three medium protocols in shoot formation (88.3%), while insignificant differences were found between the other three medium protocols. The medium protocol A was highly significantly superior to the other three medium protocols in vitrification (79.99%), while insignificant differences were found between the other three medium protocols.