Abstract
In the current study, the cytotoxic effects induced by the given photosensitizer (PS) on human rhybdomyosarcoma cancer cells (RD) as an experimental model were investigated. The experimental results like cytotoxic effects induced by the given PS on RD cells were dose dependent and the optimum concentration of PhotofrinA (R) (100 mu g/ml) along with 120 J/cm(2) generates the maximum loss in cell viability which is almost 82%. The significant loss in cell viability is the result of interaction of suitable dose of laser light (630 nm of wavelength) with PhotofrinA (R), after excitation of PS, production of reactive oxygen species (ROS), which leads to mitochondria damage and resulting of cell death (cell necrosis/cell apoptosis). Viability of controlled and treated RD cells with optimum dose of light (630 nm) has been assessed by neutral red assay (NRA) and cell damaging effect were verified by staining of mitochondria using MitotrackerA (R) red as an efficient dye as well as reactive oxygen species (ROS) accumulation detection.