Abstract
The goal of this study is to see how a sublethal dosage of sodium fluoride with and without chitosan affects cardiac function indicators and the role of chitosan as an antioxidant material. These activities were seen in albino rats using a variety of biochemical markers and confirmed by DNA and RNA analysis. Twenty-eight adult males were divided into four groups at random (n = 7), also the animals were fed a well-balanced commercial diet. The effect of chitosan nanoparticles in reducing fluoride ions toxicity in the rat heart by loading them on the Cs NPs surface to generate a physiologically suitable composite (Cs@NaF). Different methods such as field emission scanning electron microscopy (FEG-SEM), UV–Vis spectroscopy (UV–Vis), and x-ray diffraction (XRD) were used to describe the produced nanocomposite.
The following procedures can be summarized from the present research:
- Cs@NaFprotected rats by avoiding changes in lipid peroxidation in the organ, and the increased oxidative stress index shows enhanced intracellular ROS generation caused by NaF, which interacts with macromolecules in cells, including DNA, causing oxidative DNA damage.
- Heart DNA and RNA concentration in treated rats with Cs, NaF were decreased while pre-administration of Cs@NaF treated rats the concentration of DNA and RNA were partially recovered.
- NaF increased blood cholesterol, triglycerides, LDL, and VLDL levels while decreasing HDL, while the alterations in rat serum lipids were mitigated by pre-administration of Cs@NaF-treated rats.
Cs@NaF hybrid nanocomposite is recommended as an alternative because the presence of Cs NPs helped to mitigate some of the negative effects of fluoride therapy.
[Display omitted]
•Reduction in DNA and RNA concentration via Chitosan-fluoride in treated rats.•Chitosan-fluoride alleviated rat serum cholesterol, triglyceride, LDL, and vLD levels.•DNA fragmentation reduced with treated chitosan-fluoride.•Reduction of GSH concentration and catalase activity with chitosan-fluoride.•Chitosan-fluoride is stronger impact on superoxide and hydroxyl radicals.