Abstract
Novel hybrid nanocomposites have been designed, fabricated, and characterized successfully, that fabricated via a decoration of graphene oxide nanosheet surface (GO) by the nanoparticles (NPs) via the sol-gel and ultrasonication methods. Hybrid nanocomposites utilized for improving and increasing the production of fish wealth and return economically via performance improvement of the aquatic environment and assess the effects of dietary on the aquaculture system such as physiological aspects, growth, immune, enzymatic activities, and disease resistance. 1 mg/kg of examined nanocomposites used to feed the tilapia that classified into four groups each group has 15 fish/aquarium which has an average weight of about 15.03 +/- 0.04 g, the experiment performed at eight weeks. Enhanced antioxidant activity and physiological aspects via evaluation of gene expression of tilapia liver and spleen SOD tissues, that fed on fabricated nanocomposites for three months, also histo-morphology of tilapia tissue was examined. The antioxidant ability of GO@Se and GO@Se.ZnO nanocomposite due to the potent antioxidant capacity of selenium nanoparticles and zinc nanoparticles can be an excellent alternative to conventional zinc in feeding. The histopathological finding revealed severe degeneration in in-testinal villi at 24 h and liver at a one-week post-challenge with Streptococcus iniae (S. iniae) gram-positive bacteria in all groups.