Abstract
The involvement of free radicals and oxidative reactions in protein glycoxidation processes, compounds with antioxidant activity have been tested in order to reduce or to stop glycoxidation. In this study, the antioxidant potential of methonolic pericarp extract of Feronia limonia (MPFL) was evaluated using different in vitro assays including the scavenging activities of super oxide radical, hydroxyl radical, hydrogen peroxide, DPPH radical, Nitric oxide radical and Fe+3 radical scavenging activity. The extract was evaluated for antioxidant potential by the phosphomolybdenum method, Fe+2 chelating activity, Ferric Reducing Ability Power (FRAP) and 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTs) radical scavenging assay. The results indicated that the MPEF possesses the highest antioxidant activity. To establish the link between glycation and oxidation processes, further the extract have been evaluated for its in vitro antiglycation activities like the inhibitory activities on Bovine Serum Albumin (BSA) and protein oxidation markers including Protein Carbonyl Formation (PCO). The MPFL extract at different concentrations (25-100 mu g mL(-1)) has significantly quenched the fluorescence intensity of glycated BSA and the glycoxidation measured in terms of advanced glycation end products (AGEs). Furthermore, the study demonstrate that the inhibitory effects of MPFL extract in preventing oxidative protein damages including effect on PCO formation which are believed to form under the glycoxidation processes. These results clearly demonstrate that the MPFL is capable of suppressing the formation of AGEs and protein oxidation in vitro might be due to the presence of active principles like volatile flavour and free fatty acids.