Abstract
In this study, a wild-type and five distinct rifampicin-resistant (Rif
r
)
rpoB
mutants of
Pseudomonas stutzeri
(i.e., Q518R, D521Y, D521V, H531R and I614T) ability were investigated against harsh environments (particularly nutritional complexity). Among these, the robust Rif
r
phenotype of
P. Stutzeri
was associated only with base replacements of the amino deposits. The use of carboxylic and amino acids significantly increased in various Rif
r
mutants than that of wild type of
P. stutzeri
. The assimilation of carbon and nitrogen (N) sources of Rif
r
mutants’ confirmed that the organism maintains the adaptation in nutritionally complex environments. Acetylene reduction assay at different times also found the variability for N-fixation in all strains. Among them, the highest nitrogenase activity was determined in mutant ‘D521V’. The assimilation of carbon and nitrogen sources of
P. stutzeri
and its Rif
r
mutants ensures that the organism maintains the adaptability in nutritionally complex environments through fixing more nitrogen.