Abstract
Prolactin inducible protein (PIP) is a small secretary glycoprotein present in most biological fluids and contributes to various cellular functions, including cell growth, fertility, antitumor, and antifungal activities.
The present study evaluated the antibacterial activities of recombinant PIP against multiple broad-spectrum MDR bacterial strains.
The PIP gene was cloned, expressed and purified using affinity chromatography. Disk diffusion, broth microdilution, and growth kinetic assays were used to determine the antibacterial activities of PIP.
: Disk diffusion assay showed that PIP has a minimum and maximum zone of inhibition against
and
, respectively, compared to the reference drug ampicillin. Furthermore, growth kinetics studies also suggested that PIP significantly inhibited the growth of
and
. The minimum inhibitory concentration of PIP was 32 µg/mL for
(443), a standard bacterial strain, and 64 µg/mL for
sp. (LG1), an environmental multidrug-resistant (MDR) strain. The synergistic studies of PIP with ampicillin showed better efficacies towards selected bacterial strains having MDR properties.
Our findings suggest that PIP has a broad range of antibacterial activities with important implications in alleviating MDR problems.