Abstract
The main objective of this research work was to evaluate the variation in nutritional profile, antioxidant, anti-proliferative and antibacterial activities of selected species of Brassica. Five locally grown Brassica species (cauliflower, broccoli, red cabbage, white cabbage and Chinese cabbage) were collected from Ayub Agriculture Research Institute (AARI), Faisalabad, Pakistan. Polyphenolic rich extracts of these Brassicaceae species were prepared by Soxhlet extraction technique using ethanol. Phenolic acids, flavonoids and sugar contents of the investigated species were determined and quantified by RP-HPLC. Antioxidant activity was carried out by measurement of total phenolic contents (TPC), total flavonoid contents (TFC), reducing potential and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity. Anti-proliferative activity of all the extracts was determined by MTT assay on lung cancer cell line A549. Antibacterial activity was tested against the two bacterial strains, i.e., Bacillus cereus (B. cereus) and Escherichia coli (E. coli). HPLC analysis revealed the presence of gallic acid, p-coumaric acid, chlorogenic acid and benzoic acid as the major phenolic acids, whereas catechine was the major flavonoid in most of the extracts. The TPC ranged from 9.7 to 32.8 mg/g of dry plant material, measured GAE and TFC ranged from 7.7 to 23.7 mg/g of dry plant material, measured as CE. Higher TPC and TFC were found in red cabbage extract followed by cauliflower, broccoli, white cabbage and Chinese cabbage. Red cabbage extract also showed higher DPPH radical scavenging activity (IC50 = 2.3 mu g/mL) followed by cauliflower, broccoli, white cabbage and Chinese cabbage. Maltodextrose was the major sugar followed by fructose in all species of Brassica. Promising anti-proliferative and antibacterial activities were also recorded by the selected Brassica extracts.