Abstract
Type 2 diabetes mellitus (T2DM) is a chronic metabolic condition characterized by high blood glucose levels. Mutations in the WFS1 gene cause β-cell death, leading to Wolfram syndrome and diabetes. There have been few studies that have been linked to T2DM and the rs6446282 polymorphism in the WFS1 gene, and there are no studies from Saudi Arabia. As a result, the purpose of this study was to investigate the association between the rs6446482 polymorphism in the WFS1 gene and T2DM in the Saudi population.
One hundred T2DM patients and 100 healthy controls were recruited based on inclusion and exclusion criteria. Genomic DNA was obtained from collected blood, and using oligonucleotides, PCR was performed followed by RFLP analysis. Statistical analysis was performed with SPSS software to compare the numerical and categorical data.
This case-control study compared T2DM patients to healthy controls. Age, weight, BMI, FBG, HDL-c, TC, TGs, and family history (p < 0.05). The effect of the rs6446482 polymorphism was performed with PCR-RFLP analysis, and the study results confirmed the positive association with HWE (p = 0.01), genotypes (GG vs. CC: OR-3.29 [95% CI: 1.33–8.13]; p = 0.007), dominance (CC vs. CG + GG: OR-2.12 [95% CI: 1.24–3.92]; p = 0.006), and allele frequencies (G vs. C: OR-2.15 [95% CI: 1.37–3.38]; p = 0.0007), revealing a positive association. ANOVA results showed a negative association between the rs6446482 polymorphism and T2DM risk parameters such as age, weight, height, BMI and FBG.
The results of this study confirmed that the rs6446482 polymorphism is associated with T2DM in the Saudi population. This study recommends updating the meta-analysis studies in T2DM with the rs6446482 polymorphism and should also be performed in different modes of diabetes.