Abstract
The molecular phylogeny of three species of genus Buthacus, based on 16S rRNA mitochondrial DNA gene from Egypt and Saudi Arabia is presented. The inferred phylogeny based on maximum-parsimony, neighbor-joining and bayesian inference, indicates the monophyletic status of the genus Buthacus. A clear deep splitting between the "Egyptian clade" consisting of B. arenicola and B. leptochyles and the "Arabian clade" consisting of B. nigroaculatus was shown.