Abstract
Allophycocyanin (APC) belongs to a family of phycobiliproteins, extracted from Anacystis nidulans, was examined for its binding affinity towards humans genomic DNA. The genomic DNA was isolated from human's blood, stained with allophycocyanin and examined under UV trans-illuminator after electrophoresis.
Allophycocyanin dye showed high affinity towards genomic DNA. It reacted with the human genomic DNA at high dilution of >2x10(5). The allophycocyanin was seen to have no affinity towards RNA and proteins (BSA, papain). The result suggest that APC is a valuable natural dye without any biohazards and may have wide application in diagnostic studies such as fluorescent microscopy, fluorescence immunoassay, flow cytometry and fluorescence activated cell sorting and analysis.