Abstract
Vascularization and bone regeneration are closely related in the process of bone remodeling, and de-signing a bioactive scaffold with pro-angiogenic and osteogenic properties may accelerate the repair of bone defects. In this work, an iron-based metal-organic framework (MIL-88) was developed as a carrier for loading a pro-angiogenic small molecular drug (dimethyloxallyl glycine, DMOG), and then embed-ded into the PLGA nanofibrous scaffolds to repair cranial defects in rats. Imaging and histological eval-uation indicated that PLGA/MIL@D scaffold markedly enhanced vascularization and bone regeneration in vivo . Moreover, in vitro assay showed that co-delivery system significantly promoted angiogenesis by stimulating endothelial cell migration, tube formation, and enhanced osteogenesis by promoting expres-sion of osteoblast related proteins. In addition, PLGA/MIL@D scaffold promotes angiogenesis by activating the hypoxia-inducible factor-1 (HIF-1)/vascular endothelial growth factor (VEGF) signaling pathway. Al-together, this bioactive PLGA/MIL@D scaffold can combine angiogenesis with osteogenesis, and will be a bright strategy for the repair of bone defects.(c) 2023 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.