Abstract
The aim of this study was to isolate rhizobacterial strains (PGPR) that have the ability to produce phytohormones [Indol acetic acid, (IAA)], Hydrogen cyanide (HCN), Siderophore, Ammonia, Phosphate solubilization, chitinase enzyme activity and also have broad spectrum against phytpathogenic fungi. A total of thirty bacterial strains isolated from the rhizosphere of Trigonella foenum-graecum L. They are characterized according to their morphological characteristics. Twenty three bacterial isolates were found to have broad spectrum antifungal activities against pathogenic fungi, and found to be able to produce hydrogen cyanide ranging from dark brown to faint brown. Only one strain identified as Bacillus cereus KU058893 was able to produce dark brown, which indicated that it produced high amount of HCN and Brevundimonus diminuta KT277492 gave brown colour which indicate moderate production of HCN. Also, twenty bacterial isolates out of thirty with percentage 66.67% were found to be siderphore producers. Ten isolates with a percentage 33.3% were able to produce IAA in the absence of tryptophan while 66.7% were unable to produce IAA in the absence of tryptophan, B. diminuta KT277492 was the most active producer strain of IAA (24.912 +/- 0.045 mu g/ml). While B. cereus produced 15.034 +/- 0.0707 mu g/ml. While in the presence of tryptophan at 0.1% concentration, B. diminuta KT277492 was also the most active producer of IAA (31.723 +/- 0.0059 mu g/ml). While B. cereus KU058893 produced 22.2 +/- 0.046 mu g/ml. while 80% of the bacterial isolates were able to produce ammonia, 6 isolates represented 20% didn't produce ammonia. We found that about eleven isolates were able to hydrolyze Phosphate which represents about 36.667% of the total isolates, nineteen isolates were not able to hydrolysis phosphate with percentage 63.333%. B. diminuta KT277492 showed the highest chitinase enzyme activity 95 IUL-1 on the (NB+ chitin) medium.