Abstract
The entomopathogenic fungi (EPF), as a biological control agent, can provide an alternative option for high-risk insecticides.Beauveria bassianais one of the most potential EPF of pest control all over the world. The EPF isolate Y-F_ITS1 was isolated from Egyptian fauna onBeauveria-specific selective medium (BS medium). Molecular screening of theB.bassianaisolate, using PCR amplification withB. bassiana-specific primers and nucleotide sequence analysis of the internal transcribed spacer (ITS) region, confirmed the isolate asB. bassiana(accession no. [MK773644.1]). Efficacy of the isolate Y-F_ITS1 was examined against fourth instar larvae ofGalleria mellonellaL. as a model insect using concentrations from 10(4)to 10(7)spores ml(-1)in a contact toxicity assay under laboratory conditions. The pathogenicity experiment showed that all the tested concentrations caused mortalities ranged from 75 to 98.33%, within 5 days posttreatment with cumulative mortalities, reached 100%, before 7 days with typical symptoms of infection and sporulation. Further investigations are needed to prove its efficacy against different economic pests as a credible candidate of integrated pest management (IPM) program.