Abstract
Background: Very few studies described the potential effect of ischemic post conditioning (IPO) in warm hepatic ischemia. The present study hypothesizes that IPO might attenuate leucocyte infiltration. Furthermore, we aimed to compare such effect with that produced by ischemic pre conditioning (IPC), and to study the implications of vascular cell adhesion molecule-1(VCAM-1) and E selectin. Methods: 40 male Wistar rats were divided into 4 groups; group I, control group, group II ischemia reperfusion group I/R, group III underwent IPC, group IV underwent IPO. Serum levels of ALT, albumin, VCAM-1 and E selectin were determined. Liver tissue homogenate was used to assess levels of VCAM-1, E selectin and myeloperoxidase activity. Results: IPO significantly reduced serum ALT, liver VCAM-1 and E selectin levels and myeloperoxidase activity compared to I/R group, but insignificantly decreased VCAM-1 and E selectin in serum. IPO protective effects were less significant when compared to IPC group. Significant negative correlation was observed between serum E selectin and myeloperoxidase in the I/R group. Conclusion: Our results point to the protective effect of IPO in warm hepatic I/R injury through attenuation of leucocyte infiltration, and postulate that serum E selectin can be used as a marker to anticipate the degree of leucocyte infiltration in hepatic I/R injury. [Zienab Alrefaie and Laila Rashed. Leucocyte Infiltration in Experimental Warm Hepatic Ischemia Reperfusion; Effect of Ischemic Pre and Post Conditioning; Implications of Adhesion Molecules. Life Sci J 2012; 9(3): 2290-2295] (ISSN: 1097-8135). http://www.lifesciencesite.com.328