Abstract
We evaluated the efficacy of antimicrobial photodynamic therapy (A-PDT) in the reduction of pain intensity and performed cytokine profiling and measurement of microbiological parameters in patients with acute pericoronitis.
Sixty patients with age range 14-19 years were randomized into two groups: 'A-PDT group' which received additional A-PDT with conventional protocol, and 'conventional protocol group' (CPG) without A-PDT. Present pain intensity (PPI) were investigated using McGill Pain Questionnaire (MPQ) and Visual Analogue Scale (VAS). Gingival crevicular fluid were sampled to evaluate the levels of interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α) and quantified using enzyme-linked immunosorbent assay. Microbial plaque was collected to evaluate total counts of Porphyromonas gingivalis and Tannerella forsythia expressed as log colony forming unit (CFU)/mL. Level of pain intensity, cytokine profiling and microbial load were assessed at baseline (B0), and post treatment F
(Day 7) and F
(Day 14).
A total of 59 patients (30 CPG and 29 A-PDT) completed the clinical trial. Present pain intensity scores statistically significantly reduced from B
to F
in both treatment groups (p < 0.05). For VAS, both groups showed statistically significant reduction in pain scores at F
only when compared with baseline (p < 0.05). There was no inter-group significant difference at any time point for either pain scores (p > 0.05). Only TNF-α showed statistically significant reduction with A-PDT compared to CPG at both F
and F
. CPG group showed significant reduction for only T. forsythia at both follow-up points. The microbial counts for P. gingivalis and T. forsythia significantly reduced in A-PDT groups compared to CPG group at both F
and F
.
The results of this study indicate that A-PDT may be a reliable treatment option in pericoronitis for the reduction of TNF-α and microbial load. However, A-PDT does not have any effect in the reduction of pain.