Abstract
Background RNA editing is common in terrestrial plants, especially in mitochondria and chloroplast. In the photosynthesis process, NAD dehydrogenase plays a very important role. Subunit 2 of NADH-dehydrogenase is one of the major subunits in NAD dehydrogenase complex. Using desert plantCalotropis(Calotropis procera), this study focuses on the RNA editing activity of ndhB based on light time. Results NdhB(NADH-dehydrogenase subunit 2) gene accession no. MK144329 was isolated fromCalotropis proceragenomic data (PRJNA292713). Additionally, using RNA-seq data, the cDNA of thendhBgene ofC. procerawas isolated at three daylight periods, i.e., dawn (accession no. MK165161), at midday (accession no. MK165160), and pre-dusk (accession no. MK165159). Seven RNA editing sites have been found in several different positions (nucleotide no. C467, C586, C611, C737, C746, C830, and C1481) within thendhBcoding region. The rate of these alterations was deferentially edited across the three daylight periods. RNA editing rate ofndhBgene was highest at dawn, (87.5, 79.6, 78.5, 76, 68.6, 39.3, and 96.9%, respectively), less in midday (74.8, 54.1, 62.6, 47.4, 45.5, 47.4, and 93.4%, respectively), and less at pre-dusk (67, 52.6, 56.9, 40.1, 40.7, 33.2, and 90%, respectively), also all these sites were validated by qRT-PCR. Conclusion The differential editing of chloroplastndhBgene across light periods may be led to a somehow relations between the RNA editing and control of photosynthesis.