Abstract
A new flavanocoumarin, loranthin (1) together with catechin (2), quercetin (3) rutin (4), gallic acid (5) and methyl gallate (6) were isolated from Plicosepalus acacia. Loranthin possesses the rare flavanocoumarin skeleton in which the flavan and coumarin moieties are linked via C-7/C-8 of both moieties. The flavan moiety in 1 was found to be catechin, while a 3,5,6,7-tetrahydroxycoumarin fragment represented the other moiety in 1. The structures of the isolated compounds were established based on different spectroscopic data including HRFABMS, 1D and 2D NMR (COSY, HSQC, and HMBC). The free radical scavenging activities of different extracts and pure compounds were determined using DPPH reagent and all the tested samples revealed significant activities with variable percent. Loranthin exhibited a 38.4% inhibition in the free radical scavenging assay. The antimicrobial activity of loranthin was evaluated against several pathogen and loranthin displayed significant effect against Staphylococcus aureus. Moreover, the inhibition activity of the compounds against several disease-relevant protein kinases were also evaluated and discussed. (C) 2012 Phytochemical Society of Europe. Published by Elsevier B. V. All rights reserved.