Abstract
A Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain C8-1(T), was isolated from the rhizosphere soil of Nicotiana tabacum L. collected from Kunming, south-west China. The cells showed oxidase-positive and catalase-positive reactions. Growth was observed at 10-40 degrees C, at pH 6.0-8.0 and in the presence of up to 1 % (w/v) NaCl, with optimal growth at 30 degrees C and pH 7.0. The predominant isoprenoid quinone was Q-8. The major fatty acids were identified as iso-C-15 : 0 iso-C-17 : 0 and iso-C-17 : 1 omega 9c. The cellular polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, five unidentified phospholipids and two unidentified aminophospholipids. The genomic DNA G+C content was 70.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain C8-1(T) should be assigned to the genus Lysobacter. 16S rRNA gene sequence similarity analysis showed that strain C8-1(T) was closely related to Lysobacter cavernae YIM C01544(T)(98.6 %), Lysobacter soli DCY21(T) (97.6 %), Lysobacter panacisoli CJ29(T) (97.3 %), Lysobacter firmicutimachus PB-6250(T) (97.3 %), Lysobacter niastensis GH41-7(T) (97.3 %) and Lysobacter gummosus KCTC 12132(T) (97.1 %). DNA-DNA hybridization data indicated that the isolate may represent a novel genomic species belonging to the genus Lysobacter. Polyphasic taxonomic characteristics indicated that strain C8-1(T) represents a novel species of the genus Lysobacter, for which the name Lysobacter tabacisoli sp. nov. is proposed. The type strain is C8-1(T) (=KCTC 62034(T)=CGMCC 1.16271(T)).