Abstract
The aim of the current study was to develop and validate a simple, reliable and fast HPLC method for the determination of caproic acid in the methanolic extract of Vanilla planifolia using reversed phase high pressure liquid chromatography (RP-HPLC). The column used for separation was Agilent C18 Zorbax 150 x 4.6 mm, 5 mu particle size. The mobile phase used is phosphate buffer having pH of 2.45 with methanol in the ratio 40 : 60 v/v. Isocratic elution technique was used with a flow rate of 1.0 ml/min and the injection volume was 10 mu l. Detection was done using a PDA detector at a wavelength of 269 nm. The method was validated in terms of system suitability, accuracy, precision, specificity, linearity range and robustness. The method produced linear responses in the range of 50-150%. Hence a simple, reliable and rapid method was developed and validated for the estimation of caproic acid in the methanolic extract of Vanilla planifolia.