Abstract
Few redox enxymes undergo reversible electron transfer on the electrode surface primarily due to steric hindrance of the redox centers of these enzymes. Several molecular interfaces have been designed to promote electron transfer of electrode-bound redox enzymes. The authors have successfully interfaced fructose dehydrogenase and alcohol dehy- drogenase on the electrode surface with conducting polymer of polypyrrole, which could cause these enzymes to make an electron transfer with retaining their enzymatic activity. The molecular-interfaced redox enzymes will find their application in fabricating biosensors that respond specifically to the corresponding substrates in current.